Subject: Chemical sciences
Subject: Clinical medicine
Year: 2023
Type: Conference or Workshop Item
Type: NonPeerReviewed
Title: Square-wave voltammetry of human blood serum
Author: Kokoskarova, Pavlinka
Author: Lazarova, Sanja
Author: Ruskovska, Tatjana
Abstract: The study concerns on the analysis of human blood serum by using square-wave voltammetry (SWV) as a working technique. By exploring the SWV as the primary voltammetric technique, best results have been obtained with Edge Plane Pyrolytic Graphite Electrode (EPPGE) in comparison with the other electrodes used (Glassy Carbon or Pt electrodes). In the electrochemical cell, a volume of 100-1000 μL of serum without any treatment has been added into 10 ml of phosphate buffer with pH=7.34. Despite the complexity of the serum as a medium, by using the EPPGE as a working electrode, three well-defined peaks increase proportionally. This indicates that there is adsorption of uric acid (UA), bilirubin (BLR) and albumin (ALB) going on to the surface of the working electrode. In addition, the parabolic dependence of the measured peak-currents of all three peaks as a function of the SW frequencies have been observed. This phenomenon is known as “quasi-reversible maximum” and it is typical for systems undergoing an electrochemical transformation of the working electrode from an adsorbed state. The effect of some interferences of organic systems present in the human serum have been also monitored. The major advantage of the method is that it is fast, inexpensive, and it does not require treatment of the sample, as it is sensitive to micromolar concentrations. The major disadvantage of the explored methods is that it requires very frequent electrode cleaning due to the adsorption of compounds present in the human serum.
Publisher:
Relation: https://eprints.ugd.edu.mk/35815/
Identifier: oai:eprints.ugd.edu.mk:35815
Identifier: https://eprints.ugd.edu.mk/35815/1/ilovepdf_merged.pdfIdentifier: https://eprints.ugd.edu.mk/35815/3/poster%20Lodz%20PK.pdfIdentifier: Kokoskarova, Pavlinka and Lazarova, Sanja and Ruskovska, Tatjana (2023) Square-wave voltammetry of human blood serum. In: XIV Sesja Magistrantów I Doktorantów Łódzkiego Środowiska Chemików. (Unpublished)
